qPCR & NGS Workflows

Products

qPCR WORKFLOW

Our integrated qPCR workflow is highly automated, freeing up valuable resources and reducing turn-around time for lab operators. By offering a flexible system that runs multiple tests on the same platform, simultaneously runs up to four tests in parallel, and identifies several targets in the same sample in the same run, laboratory instrumentation complexity is reduced. Additionally, open-channel capability allows adaptation to lab-specific needs.

Sentosa SX101

A flexible automated pipetting system that offers multipurpose utility for sample lysis, nucleic acid extraction and PCR set-up as well as for NGS library prep.

Rotor-Gene Q

Using an innovative rotary design, the Rotor-Gene Q MDx 5plex HRM Platform allows for unmatched thermal performance in real-time PCR cycling. The use of a rotating disc ensures that every single tube is spun in a chamber of moving air that precisely keeps temperatures uniform. This allows for rapid thermal cycling and temperature precision across all samples.

Sentosa SA Reporter

The Sentosa SA software automatically analyzes results obtained from the Rotor-Gene Q instrument. The innovative analytics interprets even complex sequencing data to produce comprehensive, consise reports.

Tests

Freshstart Medical offers real-time polymerase chain reaction (qPCR) tests for the detection and/or quantitation of a wide range of viruses and gene mutations. The flexibility of the platform allows for consolidated testing with a range of sample types.

Zoonotic Diseases

MPXV

Bloodborne Viruses

HBV Quant

Microbiology

Direct MRSA/SA
UTI ID & AMR

Human Herpes and Polyomaviruses

BKV Quant
CMV Quant
EBV Quant
HSV-1/2 QUant
HSV-1/2 Qual
VZV Quant

Leukemia

BCR-ABL(M)Qt
BCR-ABL(m)Qt

Respiratory Viruses

Influenza A/B & RSV
SARS-CoV-2 v1.0
SARS-CoV-2 v2.0
SARS-CoV-2 ID
Flu/RSV/SARS-CoV-2

Additional Products

Extraction Kits

Bacterial gDNA
FFPE gDNA
Total RNA
Virus Total NA
Virus Total NA Plus
Whole Blood

Labware

Consumables

NGS WORKFLOW

Our NGS solution is easy-to-use, from automated template preparation and library construction, to sequencing, data analysis and reporting. The automated solution reduces sample-to-sample variability, minimizes risk of rerun due to user error/inconsistency, and help produce more accurate results. The open platform allows for adaptation to personalized workflows.

Sentosa SX101

A flexible automated pipetting system that offers multipurpose utility for sample lysis, nucleic acid extraction and PCR set-up as well as for NGS library prep.

Sentosa SQ301

The Sentosa SQ301 is simple, fast and affordable, putting the power of NGS technology in the reach of labs of all sizes. Using semi-conductor based sequencing technology, it is ideal for sequencing targeted regions. The system allows for parallel analysis of up to 16 sample barcodes.

Sentosa SQ Reporter

The Sentosa SQ Reporter software automatically analyzes results obtained from the Sentosa SQ 301 instrument. The innovative analytics interprets even complex sequencing data to produce comprehensive, concise reports.

Tests

Several NGS assays are being developed to identify sequence variants of demonstrated importance, including gene mutations relevant to oncology and viral genotypes, using a variety of sample types.

Oncology

NSCLC
OncoKey Core
OncoKey Focus
OncoKey Hereditary
OncoKey SL 60 Plus
OncoKey SL 525 Plus

Virology

HCV
HIV/HCV/HBV/CMV FLEX
SARS-CoV-2 FLEX

Microbiology

Pathogen ID

Additional Products

Freshstart Medical offers a broad portfolio of extraction kits for the isolation of DNA and RNA from various sample types. Reagents are optimized for reliable performance in conjunction with the Vela Diagnostics qPCR and NGS test portfolio. Low sample input amounts and the inclusion of an extraction control are features of all Freshstart Medical extraction kits.

Library Prep

Fragm Library Prep

Sequencing

318 Chip
Sequencing Kit
Template Kit

Extraction Kits

FFPE II gDNA

Labware

Consumables

Increasing
Cost Effectiveness

Laboratories can cost-effectively generate accurate, reliable results with qPCR and NGS solutions from Freshstart Medical.

The utilization of common instrumentation across both qPCR and NGS technologies reduces equipment and service costs, as well as minimizes footprint. Automated workflows mean less dependence on hands-on time, freeing personnel for more value-added tasks.

Freshstart Medical developed a test portfolio that minimizes costs associated with generating results. The ability to run multiple tests on the same platform, running tests in parallel in the same run, and testing for several targets in the same eluate are all ways Freshstart Medical helps laboratories gain economic efficiencies.

Providing Laboratory Efficiency

The Sentosa system from Freshstart Medical provides standardized versatility from sample input to result upload. The multipurpose Sentosa SX101 performs automated lysis, nucleic acid extraction and PCR set-up on a wide range of sample types;  it can also be used for NGS library preparation.  Using shared instrumentation, customers can enjoy the flexibility of harnessing either or both technologies in research areas that require comprehensive testing, to accurately and efficiently provide test results.  With open-channel capabilities, laboratories can also run user-defined protocols.

The option of generating concise reports using Sentosa Reporter for qPCR and NGS results is readily available.

The Freshstart Medical qPCR and NGS workflows maximize efficiency by:

Requiring minimal sample input volume

Reducing time to result and automated reporting

Combining detection of common targets in the same run

Automating processes from sample to result

KNOWLEDGE, EXPERIENCE AND
FRESH IDEAS.

With automated solutions for both real-time PCR (qPCR) and Next-Generation Sequencing (NGS) workflows using common instrumentation, Freshstart Medical challenges convention to meet different customer demands.

Freshstart Medical uniquely combines qPCR with NGS workflows to provide laboratories with increased efficiency and effectiveness. With RNA and DNA extraction, qPCR assays that detect a wide variety of viruses and gene mutations, and NGS solutions* for identifying viral genotypes and sequence variants, Freshstart Medical helps drive scientific discoveries today for improvements tomorrow.

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LABORATORY SOLUTIONS

COMBINED AUTOMATED PCR AND NGS WORKFLOWS

COMBINED AUTOMATED PCR AND NGS WORKFLOWS

EXPLORE LABORATORY SOLUTIONS

We pay very close attention to detail when integrating applications between each platform.