Rotor-Gene® Q MDx

Automated NGS data analysis and sample reporting

Overview

Using an innovative rotary design, the Rotor-Gene® Q MDx 5plex HRM Platform allows for unmatched thermal performance in real-time PCR cycling. The use of a rotating disc ensures that every single tube is spun in a chamber of uniform temperature, using air as the conducting element. This allows for rapid thermal cycling and precise results in all tubes.

For detection, a set of 6 uncoupled excitation and emission filters are used in conjunction with 6 dedicated LEDs to maximize the detection capability. Each tube is illuminated by the excitation LED and the fluorescent signal transmitted to the PMT detector via a uniform optical path length. This eliminates well-to-well optical variation due to edge effect which block-based cycler had experienced. It also allows for fluorescent detection without the need of a reference dye.

With thermal and optical uniformity, the Rotor-Gene® Q MDx 5plex HRM Platform offers unparalleled quantitative and genotyping analysis for PCR applications.

Ordering Information

Rotor-Gene® Q MDx

Item Number: 400046

Pack Size: 1



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    OPTICAL RANGE

    With 5 channels offering detection from the green to infrared wavelengths, the Rotor-Gene® Q MDx platform provides wide optics for most fluorophores available in the market. The decoupled filter sets create flexibility for customized excitation/detection wavelength combinations, making the platform compatible with most fluorophores.

    Channel
    Excitation (nm)
    Detection (nm)
    Examples of fluorophores detected

    Green

    470±10

    510±5

    FAM, YYBR Green I, Fluorescein, EvaGreen, Alexa Fluor 488

    Yellow

    530±5

    557±5

    JOE, VIC, HEX, TET, MAX, CAL Fluor Gold 540, Yakima Yellow

    Orange

    585±5

    610±5

    ROX, CAL Fluor Red 610, Cy 3.5, Texas Red, Alexa Fluor 568

    Red

    625±5

    660±10

    Cy5, Quasar 670, LightCycler Red640, Alexa Fluor 663

    Crimson

    680±5

    712 high pass

    Quasar 705, LightCycler Red 705, Alexa Fluor 680

    High Resolution Melt (HRM)

    460±20

    510±5

    SYBR Green I, SYTO 9, LC Green, LC Green Plus+, EvaGreen

    FEATURES AND BENEFITS

    Supports a wide range of assays such as Melanoma, HCV Genotyping, Ion Ampliseq Cancer Hot Spot (Life Technologies), thereby minimizing the need for different solutions for different assays
    Secondary analysis in as little as four hours on 318 chip ensures a fast turn-around time
    High level quality management:
    Supplies easy-to-interpret QC and reports for each sample. The QC report contains statistics such as throughput, coverage and chip loading. The report contains a summary of the results together with assay and sequencing information.
    E-signature approval system tracks record approval by techicians and lab directors and supports up to five levels of usrs. All signtificant events aree logged with timestamp, user account information and IP address.

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